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viability assay kit  (Biotium)


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    Structured Review

    Biotium viability assay kit
    Viability Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viability+cytotoxicity+assay+kit/Viability%2FCytotoxity+Assay+Kit+for+Animal+Live+%26+Dead+Cells/pmc13124311-195-15-18
    Average 95 stars, based on 110 article reviews
    viability assay kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Electrostatic Patterning of Nanofibrous Microcapsules for Three-Dimensional Cell Culture
    Article Snippet: .. For visualization of cell viability, live/dead staining was performed following the 96 h culture period using Viability/Cytotoxicity Assay Kit (No.30002, Biotium, Fremont, CA, USA) consisting of Calcein-AM (live cells, green) and ethidium homodimer-1 (dead cells, red) according to the manufacturer’s protocol. .. Live-cell imaging was carried out using confocal laser scanning microscopy (FV-1200, Olympus, Tokyo, Japan) following Calcein-AM staining, enabling three-dimensional visualization of viable cells and assessment of their spatial distribution within the scaffold.

    Article Title: Prospective Assessment of Embryoid Body by Deep Learning on Label-Free Time-Lapse Images from the Microwell Array
    Article Snippet: .. After 24 h, the culture medium was replaced with a culture medium that did not contain Y27632, and then the medium was partially replaced in half every 24 h. To ensure precise measurement of viable areas, EBs were stained after 72 h using the Viability/Cytotoxicity Assay Kit (No. 30002, Biotium, Fremont, CA, USA), which contains Calcein-AM for identifying live cells (green fluorescence) and ethidium homodimer-1 for detecting dead cells (red fluorescence), in accordance with the manufacturer’s instructions. ..

    Article Title: Electrostatic Patterning of Nanofibrous Microcapsules for Three-Dimensional Cell Culture.
    Article Snippet: .. For visualization of cell viability, live/dead staining was performed following the 96 h culture period using Viability/Cytotoxicity Assay Kit (No.30002, Biotium, Fremont, CA, USA) consisting of Calcein-AM (live cells, green) and ethidium homodimer-1 (dead cells, red) according to the manufacturer’s protocol. .. Live-cell imaging was carried out using confocal laser scanning microscopy (FV-1200, Olympus, Tokyo, Japan) following CalceinAM staining, enabling three-dimensional visualization of viable cells and assessment of their spatial distribution within the scaffold.

    Article Title: Alternative Approach to the Synthesis of Vinyl Ester Resins—Composites and Their Biomedical Application
    Article Snippet: .. The viability of bacterial cells and their adhesion to the sample surfaces were assessed using double fluorescent staining for both live and dead bacteria with the Viability/Cytotoxicity Assay kit for Bacteria LIVE/DEAD Cells (Biotium, Hayward, CA, USA) [ ]. ..

    Article Title: Prospective Assessment of Embryoid Body by Deep Learning on Label-Free Time-Lapse Images from the Microwell Array
    Article Snippet: .. After 24 h, the culture medium was replaced with a culture medium that did not contain Y27632, and then the medium was partially replaced in half every 24 h. To ensure precise measurement of viable areas, EBs were stained after 72 h using the Viability/Cytotoxicity Assay Kit (No. 30002, Biotium, Fremont, CA, USA), which contains Calcein-AM for identifying live cells (green fluorescence) and ethidium homodimer-1 for detecting dead cells (red fluorescence), in accordance with the manufacturer’s instructions. ..

    Fluorescence:

    Article Title: Prospective Assessment of Embryoid Body by Deep Learning on Label-Free Time-Lapse Images from the Microwell Array
    Article Snippet: .. After 24 h, the culture medium was replaced with a culture medium that did not contain Y27632, and then the medium was partially replaced in half every 24 h. To ensure precise measurement of viable areas, EBs were stained after 72 h using the Viability/Cytotoxicity Assay Kit (No. 30002, Biotium, Fremont, CA, USA), which contains Calcein-AM for identifying live cells (green fluorescence) and ethidium homodimer-1 for detecting dead cells (red fluorescence), in accordance with the manufacturer’s instructions. ..

    Article Title: Prospective Assessment of Embryoid Body by Deep Learning on Label-Free Time-Lapse Images from the Microwell Array
    Article Snippet: .. After 24 h, the culture medium was replaced with a culture medium that did not contain Y27632, and then the medium was partially replaced in half every 24 h. To ensure precise measurement of viable areas, EBs were stained after 72 h using the Viability/Cytotoxicity Assay Kit (No. 30002, Biotium, Fremont, CA, USA), which contains Calcein-AM for identifying live cells (green fluorescence) and ethidium homodimer-1 for detecting dead cells (red fluorescence), in accordance with the manufacturer’s instructions. ..

    Bacteria:

    Article Title: Bio-Derived Cellulose Nanofibers for the Development Under Environmentally Assessed Conditions of Cellulose/ZnO Nanohybrids with Enhanced Biocompatibility and Antimicrobial Properties.
    Article Snippet: Luria–Bertani broth (LB) was obtained from MP Biomedicals (Illkirch, France). .. Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead https://doi.org/10.3390/ma19020346 Cells was purchased from Biotium (Hayward, CA, USA). .. L-glutamine and antibiotics were obtained from Gibco (New York, NY, USA).

    Article Title: Alternative Approach to the Synthesis of Vinyl Ester Resins—Composites and Their Biomedical Application
    Article Snippet: .. The viability of bacterial cells and their adhesion to the sample surfaces were assessed using double fluorescent staining for both live and dead bacteria with the Viability/Cytotoxicity Assay kit for Bacteria LIVE/DEAD Cells (Biotium, Hayward, CA, USA) [ ]. ..

    Article Title: Bio-Derived Cellulose Nanofibers for the Development Under Environmentally Assessed Conditions of Cellulose/ZnO Nanohybrids with Enhanced Biocompatibility and Antimicrobial Properties
    Article Snippet: Luria–Bertani broth (LB) was obtained from MP Biomedicals (Illkirch, France). .. Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead Cells was purchased from Biotium (Hayward, CA, USA). .. L-glutamine and antibiotics were obtained from Gibco (New York, NY, USA).



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    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
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    Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    Cell-scaffold interaction <t>(A)</t> <t>Live/Dead</t> staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. <t>(N,</t> <t>O)</t> <t>Calcein-AM/PI</t> flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. <t>(N,</t> <t>O)</t> <t>Calcein-AM/PI</t> flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. <t>(N,</t> <t>O)</t> <t>Calcein-AM/PI</t> flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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    Image Search Results


    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

    Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control

    Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Ready-to-use 3D bioprinted scaffolds from natural materials loaded with patient’s PRGF for personalized skin regeneration

    doi: 10.1016/j.isci.2026.116185

    Figure Lengend Snippet: Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: LIVE/DEAD® viability/cytotoxicity kit , Fisher Scientific , Cat# L3224.

    Techniques: Staining, Imaging

    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Rational design of FAP-targeted sEVs delivered by microneedles for precision treatment of hypertrophic scars via ferroptosis in hypertrophic scar fibroblasts

    doi: 10.1016/j.mtbio.2026.103117

    Figure Lengend Snippet: In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: Cell viability and death were assessed using a Calcein-AM/PI Cell Viability/Cytotoxicity Kit (Beyotime, China).

    Techniques: In Vitro, Staining, CCK-8 Assay, Migration, Wound Healing Assay, Western Blot, Control, Immunofluorescence, Fluorescence, Flow Cytometry